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KMID : 0613320010070020041
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Journal of the Korean Bone and Joint Tumor Soceity
2001 Volume.7 No. 2 p.41 ~ p.50
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Biochemical Markers for Osteosarcoma
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Lee Chang-Woo
Cho Woo-Jin
Cho Jae-Lim
Kim Tai-Seung
Hwang Kun-Sung
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Abstract
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Purpose: To investigate biochemical markers for osteosarcoma, activities of deoxyribocucle-ase(DNase), ribonuclease(RNase), 5’-nucleotidase, alkaline phosphatase and amylase were determined in the osteosarcoma tissue and serum of patients with osteosarcoma. Also studied were DNase, RNase in osteosarcoma tissue, isolating the enzymes from the sarcoma tissue and investigating the sarcoma specific enzymes.
Materials and Methods: The experimental tissue and serum were obtained from twelve patients with osteosarcoma. The control group were obtained from the normal healthy tissue of the same patients. The tissue were centrifugalized to obtain extracts. The extracts were analized for the estimation of nucleic acid, protein contents and enzyme activities. And then each enzymes were isolated and analized by DEAE-cellulose chromatography and estimated for activities.
Result: Activities of acid DNase, RNase, 5’-nucleotidase and alkaline phosphatase were sig-nificantly increased in osteosarcoma tissue. Neutral RNase in osteosarcoma tissue was shown to bo highly active, exhibiting secretory form of RNase inhibitor associated with the RNase was also increased. In the serum of patients with osteosarcoma, RNase activity was significantly increased. DEAE-cellulose column chromatographical analysis revealed that acid DNase was isolated as a single enzyme and neutral RNase as five isozymes in osteosarcoma tissue.
Conclusion: The results indicated that combination of these enzymes could be used as mark-ers for osteosarcoma. The results indicated that acid DNase and neutral RNase might play a role in genesis of sarcoma and suppression of sarcoma.
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KEYWORD
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Osteosarcoma, Biochemical marker
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